List of top Biotechnology Tools and Techniques Questions asked in GAT-B

Given below are two statements:
Statement I: Precision refers to the closeness of various measurements for the same quantity.
Statement II: Accuracy is the agreement of the obtained value with the known or true value of the quantity.
In light of the above statements, choose the correct answer from the options given below:

Which one of the following is NOT true for Quantum dots as fluorescent probes in fluorescence microscopy?
The microscope which uses lasers to scan the specimen at a specific depth, illuminates one area at a time and blocks stray light to give an image with excellent contrast and resolution is
Which of the following is normally used to determine the frequency of interferon gamma producing cells in a blood sample?
The distance between each turn in the helical strand of DNA is
Which of the following biochemical techniques does not use antibody?
A culture of E. coli cells (1 ml) was diluted \(10^6\) fold and 100 $\mu$l of this was used for plating. After 24 hours incubation, the number of colony forming units (CFU) was found to be 180. The CFU count of the original culture is
Which of the following DNA, in its double-stranded form, will have the highest \(T_m\)?
Which of the following is generally NOT a step in PCR?
The purity of a plasmid DNA preparation is determined by analyzing the ratio \(A_{260}/A_{280}\). In a pure DNA preparation, the ratio will be
Which of the following exhibits maximum mobility in an agarose gel?
Which of the following is NOT a multiple sequence alignment program?
EST stands for
Given below are two statements: Statement I: Protein-ligand docking involves binding of a small molecule to the protein receptor. Statement II: Both AUTODOCK and LUDI are protein-ligand docking programs. In the light of the above statements, choose the correct answer from the options given below:
Which of the following could be a recognition site of a Type II restriction endonuclease?
Which one of the following is incorrect about DNA microarray?
Match List - I with List - II.
Choose the most appropriate match from the options given below:
Which of the following statements is NOT TRUE about myeloma cells used in the hybridoma technique for the production of monoclonal antibodies?
A plant species nearing its extinction due to virus infection has been given for tissue culture. What should scientists choose from the following four explants for culturing?
Given below are two statements:
Statement I: The recombination frequency of genes vary from 50% to 80%.
Statement II: Recombination can occur anywhere along the length of chromosome.
Match items in List I with items in List II
List I: A. Silent mutation, B. Missense mutation, C. Nonsense mutation, D. Frameshift mutation
List II: I. Amino acid codon changed to stop codon, II. Sequence beyond mutation changed, III. Sequence changed in one amino acid, IV. Amino acid sequence is unchanged
In Michaelis-Menten kinetics to increase the reaction velocity by 9 fold from 10% of $V_{max}$ to 90% of $V_{max}$ the substrate concentration has to increase by:
Match items in List I with items in List II
List I: A. Rickettsia prowazekii, B. Variola major, C. Varicella zoster Virus, D. Borrelia recurrentis
List II: I. Relapsing fever, II. Epidemic typhus, III. Small pox, IV. Shingles
Identify the statement that is NOT applicable to enzyme catalyzed reaction.
Subtractive hybridization is useful to: