List of top Biotechnology Tools and Techniques Questions asked in GAT-B

Recombinants with insertional inactivation of $\beta$-galactosidase are identified by their inability to:
The level of expression of recombinant protein can be gauged by:
The percentage of sequence identity for the following aligned sequence is:
V K S F L W T Q A L
V P S F R W T Q S L
During replication, DNA polymerases distinguish between ribonucleoside and deoxyribonucleoside triphosphates (rNTPs and dNTPs) while synthesizing new DNA strand. This is because:
The following figure shows agarose gel electrophoresis of linear DNA, after digestion with BamHI in lane C and EcoRI in lane D. The uncut DNA is shown in lane B.
Statement I: This DNA has two restriction sites for BamHI and one restriction site for EcoRI.
Statement II: Double digestion with BamHI and EcoRI will yield 5 fragments.
Regulation of lac operon is negative as:
The following diagram represents a restriction enzyme site:
Statement I: After digestion cohesive ends will be formed.
Statement II: These cohesive ends will have phosphate groups at 3' and 5' ends.
Which of the following can be a restriction site for type II restriction enzyme?
A double-stranded genomic DNA has 40% (A+T) content. What is the average size (base pairs) of the fragments generated by digestion with the restriction enzyme HaeIII (GGCC)?
Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R.
Assertion A: Attenuation of the trp operon is possible in bacteria.
Reason R: Transcription and translation are coupled.
Lac repressor of E. coli contains ________ DNA binding motifs.
Which protein is required to seal a nick during Okazaki fragment maturation?
Self splicing introns are an example of:
Given below are two statements about hnRNA processing:
Statement I: Capping occurs by addition of methyl adenosine triphosphate to the 5' end.
Statement II: During tailing, about 200-300 adenylate residues are added to its 3' end.
Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R.
Assertion A: A linear DNA molecule containing three recognition sites for a restriction enzyme will generate multiple DNA fragments on partial digestion.
Reason R: Partial digestion by restriction enzymes leads to a heterogeneous population of DNA fragments.
Match items List I with items in List II:
Given below are two statements:
Statement I: The fragments of DNA generated after cleavage with a restriction enzyme have a phosphate group on their 3' ends and a -OH group on their 5' ends.
Statement II: Cohesive ends generated by restriction digestion reanneal with complementary DNA fragment.
Simple sequence repeats (SSRs) are best utilized for:
How many clones must you get to make a representative genomic library of a plant with a genome of a billion base pairs (bp), provided your vector carrying capacity is 10 kbp. Remember to allow a redundancy of at least one order of magnitude.
Which one of the following techniques can be used to determine the copy number of a gene?
Which enzyme catalyzes strand separation at the replication fork?
Which one of the following statements correctly describes type IIP restriction enzymes?
A. They cut nucleotides only from the ends of the DNA.
B. They act on only palindromic nucleotide sequences in the DNA.
C. They recognize only GC rich sequences.
D. They recognize only AT rich sequences.
One of the limitations of Y-chromosome DNA profiling is that
Match the enzymes in the List I to their respective functions in the List II.
An unknown solution shows distinct peaks at 260 and 280 nm in a UV spectrophotometric analysis. The solution may contain