Question:
From the purification table, what is the overall protein yield?
From the purification table, what is the overall protein yield?
Show Hint
Yield tracks the "Activity" you kept, not the "Protein" mass you threw away.
Updated On: May 14, 2026
- 100%
- 20.70%
- 45%
- 70%
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The Correct Option is C
Solution and Explanation
Step 1: Concept
Protein yield is calculated as: $(\text{Total Activity in Final Step} / \text{Total Activity in Crude Extract}) \times 100$.
Step 2: Analysis
Initial Activity (Crude) = 100,000 units.
Final Activity (Affinity) = 45,000 units.
Step 3: Calculation
Yield = $(45,000 / 100,000) \times 100 = 45%$.
Step 4: Conclusion
The overall yield is 45%. Note: While the text says 20.70% based on the key (5610), the provided data ($45k / 100k$) results in 45%. Final Answer: (C)
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