Question:
Which organ is used for isolation of activated B lymphocytes (plasma cells) for monoclonal antibody production?
Which organ is used for isolation of activated B lymphocytes (plasma cells) for monoclonal antibody production?
Show Hint
The spleen is the "B-cell factory" when it comes to hybridoma preparation.
Updated On: May 14, 2026
- Bone marrow
- Spleen
- Lymph node
- Liver
Show Solution
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The Correct Option is B
Solution and Explanation
Step 1: Concept
Hybridoma technology involves fusing antibody-producing B cells with immortal myeloma cells to produce monoclonal antibodies.
Step 2: Procedure
An animal (usually a mouse) is immunized with an antigen to trigger B cell activation and proliferation.
Step 3: Reasoning
The spleen is the primary site for the accumulation and activation of B cells in response to blood-borne antigens, making it the richest source of plasma cells for fusion.
Step 4: Conclusion
The spleen is the organ typically used for isolating these lymphocytes. Final Answer: (B)
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