A student observed a decrease in Western blotting signal for PTEN in cancer cells but no change in Northern blotting signal. Which statement is correct?
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Western = Protein. Northern = RNA. Southern = DNA.
There is a decrease in PTEN protein but no mutation in PTEN DNA
There is a decrease in PTEN protein but no change in mRNA expression
There is a decrease in mRNA expression but no change in protein level
There is a decrease in mRNA expression but no mutation in PTEN DNA
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The Correct Option isB
Solution and Explanation
Step 1: Concept
Western blotting detects specific proteins, while Northern blotting detects specific mRNA sequences.
Step 2: Analysis of Results
A decreased Western blot signal means less PTEN protein is present. No change in Northern blot signal means PTEN mRNA levels are normal.
Step 3: Reasoning
Since mRNA is normal but protein is low, the regulation issue is occurring post-transcriptionally (at the level of translation or protein degradation).
Step 4: Conclusion
There is a decrease in protein level with no change in mRNA expression.
Final Answer: (B)
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