Question:
Nick translation is carried out by
Nick translation is carried out by
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DNA Pol I is the "renovator": it tears down the old wall (exonuclease) and builds a new one (polymerase) at the same time.
Updated On: May 14, 2026
- DNA polymerase I
- RNA Polymerase II
- Ribosomes
- Polynucleotide kinase
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The Correct Option is A
Solution and Explanation
Step 1: Concept
Nick translation is a tagging technique used to label DNA with radioactive or fluorescent nucleotides.
Step 2: Meaning
The process involves creating a "nick" (a break in the phosphodiester backbone) using DNase I, followed by the action of a polymerase.
Step 3: Analysis
DNA polymerase I uses its $5' \rightarrow 3'$ exonuclease activity to remove existing nucleotides ahead of the nick while simultaneously adding new labeled nucleotides using its $5' \rightarrow 3'$ polymerase activity.
Step 4: Conclusion
Therefore, Nick translation is carried out by DNA polymerase I. Final Answer: (A)
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