A plant species nearing its extinction due to virus infection has been given for tissue culture. What should scientists choose from the following four explants for culturing?
Step 1: Concept
Virus-free plants can be regenerated even from infected individuals using specific tissue culture techniques.
Step 2: Meaning
The goal is to select tissue that the virus hasn't reached or cannot survive in.
Step 3: Analysis
The shoot apical meristem is generally free of viruses due to the absence of vascular tissue (plasmodesmata/phloem) and the high rate of cell division which outpaces viral replication. "Meristem culture" is the standard practice for eliminating viral infections in crops.
Step 4: Conclusion
Meristematic tissue provides the best chance for obtaining a virus-free clone.
Final Answer: (A)
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