Question:
Which of the following statements is NOT TRUE about myeloma cells used in the hybridoma technique for the production of monoclonal antibodies?
Which of the following statements is NOT TRUE about myeloma cells used in the hybridoma technique for the production of monoclonal antibodies?
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Hybridoma Myeloma = Immortal + HGPRT negative + Non-secretory.
Updated On: May 14, 2026
- These are cancerous B cells
- They can divide indefinitely in a culture
- Their HGPRT gene is non-functional
- They can produce antibodies
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The Correct Option is D
Solution and Explanation
Step 1: Concept
Hybridomas are created by fusing a plasma B-cell with a myeloma cell to combine specificity with immortality.
Step 2: Meaning
The myeloma cell partner must be "non-secretory" for the technique to work cleanly.
Step 3: Analysis
Statements A, B, and C are true: myeloma cells are immortal (cancerous) B-cells that lack HGPRT (enabling HAT selection). Statement D is false because the specific myeloma cell lines used in research are selected to be non-producers; they should not produce their own antibodies, ensuring the resulting hybridoma only secretes the antibody from the parent plasma cell.
Step 4: Conclusion
Non-secretion of antibodies is a requirement for a suitable myeloma fusion partner. Final Answer: (D)
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