Question

Identify the form of chromatography [Reverse Phase (RP) or Gel filtration (GF)] as shown in the chromatograms A and B below:
(A shows Protein eluting before Salt; B shows Salt eluting before Protein)

• A. RP(A) and GF(B)
• B. RP(B) and GF(A)
• C. RP(A) and RP(B)
• D. GF(A) and GF(B)

Hint:

GF = Big stuff first. RP = Polar stuff first.

Answer 1

The Correct Option is A

Step 1: Concept
Gel filtration (Size Exclusion) separates molecules by size, where larger molecules elute first. Reverse Phase separates based on hydrophobicity, where polar molecules (like salts) elute first.

Step 2: Analysis of A
In A, the large protein elutes before the small salt molecules. This is a characteristic of Gel filtration.

Step 3: Analysis of B
In B, the polar salt elutes before the non-polar protein. This is characteristic of Reverse Phase chromatography.

Step 4: Conclusion
Therefore, A is GF and B is RP. Note: While the key (5595) says RP(A) and RP(B), the principles of chromatography define A as GF and B as RP. Final Answer: (A)