List of top Biotechnology Tools and Techniques Questions asked in GAT-B

The restriction endonuclease which cleaves the given sequence at the indicated site is: $$(5') G \downarrow GATCC (3')$$ $$(3') CCTAG \uparrow G (5')$$

EcoR1 enzyme recognizes the sequence GAATTC. A stretch of linear DNA with six sites will give rise to

In Transmission Electron Microscope (TEM), a beam of electrons interact with the specimen to form the image as

Which of these techniques cannot differentiate between identical twins?

A 5200-bp vector contains two BamHI sites 450-bp apart. A 1200-bp gene "X" with BamHI sites at ends is cloned after complete digestion. Size of the resultant plasmid?

Match various nucleases in the List I to their respective functions in List II.

Nick translation is carried out by

Identify the form of chromatography [Reverse Phase (RP) or Gel filtration (GF)] as shown in the chromatograms A and B below:
(A shows Protein eluting before Salt; B shows Salt eluting before Protein)

From the purification table, what is the overall protein yield?

A student observed a decrease in Western blotting signal for PTEN in cancer cells but no change in Northern blotting signal. Which statement is correct?

Which organ is used for isolation of activated B lymphocytes (plasma cells) for monoclonal antibody production?

PSSM stands for

Papain digestion of an IgG predominantly results in the formation of