Question:
Subtractive hybridization is useful to:
Subtractive hybridization is useful to:
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Subtraction = Removing common genes to find unique ones.
Updated On: May 14, 2026
- eliminates incomplete cDNA from a gene library.
- create expression libraries based on genes that are currently being expressed.
- identify and construct new probes for southern hybridization.
- identify sets of genes that are only expressed under certain conditions.
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The Correct Option is D
Solution and Explanation
Step 1: Concept
Subtractive hybridization is used to isolate DNA/RNA sequences that are present in one sample but absent in another.
Step 2: Meaning
It effectively "subtracts" common sequences, leaving behind the unique ones.
Step 3: Analysis
By comparing the mRNA of a cell under Condition A and Condition B, this technique allows researchers to find genes specifically induced by a particular stimulus or environment. This is vital for studying differential gene expression.
Step 4: Conclusion
The technique is designed to find unique differentially expressed genes. Final Answer: (D)
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