Question:
Which protein is required to seal a nick during Okazaki fragment maturation?
Which protein is required to seal a nick during Okazaki fragment maturation?
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Ligase = Links/Seals DNA strands.
Updated On: May 14, 2026
- DNA Ligase
- DNA helicase
- DNA topoisomerase
- DNA polymerase
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The Correct Option is A
Solution and Explanation
Step 1: Concept
DNA replication on the lagging strand occurs in short segments called Okazaki fragments.
Step 2: Meaning
"Maturation" involves removing RNA primers, filling the gaps with DNA, and joining the fragments together.
Step 3: Analysis
DNA Polymerase I (in prokaryotes) replaces RNA primers with DNA, but it cannot join the 3'-OH of one fragment to the 5'-phosphate of the next. This "nick" requires the formation of a phosphodiester bond. DNA Ligase catalyzes this specific reaction using ATP or NAD+ as energy.
Step 4: Conclusion
DNA Ligase is the "glue" that seals nicks in the sugar-phosphate backbone. Final Answer: (A)
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