Question:

Which enzyme catalyzes strand separation at the replication fork?

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Helicase "unzips" the Genes.
Updated On: May 14, 2026
  • Replication protein A
  • DNA helicase
  • DNA topoisomerase
  • DNA polymerase
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The Correct Option is B

Solution and Explanation


Step 1: Concept

DNA replication requires the double helix to be unwound to expose the template strands.

Step 2: Meaning

Strand separation involves breaking the hydrogen bonds between complementary nitrogenous bases.

Step 3: Analysis

DNA polymerase synthesizes new strands, and topoisomerases relieve torsional strain. DNA helicase is the specific motor protein that moves along the phosphodiester backbone, unzipping the two strands.

Step 4: Conclusion

DNA helicase is the enzyme responsible for the mechanical separation of DNA strands at the fork. Final Answer: (B)
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