Question:

Which one of the following statements correctly describes type IIP restriction enzymes?
A. They cut nucleotides only from the ends of the DNA.
B. They act on only palindromic nucleotide sequences in the DNA.
C. They recognize only GC rich sequences.
D. They recognize only AT rich sequences.

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Type IIP: The "P" stands for Palindromic recognition.
Updated On: May 14, 2026
  • A and B Only
  • B Only
  • A, B and C Only
  • A, B and D Only
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The Correct Option is B

Solution and Explanation


Step 1: Concept

Type II restriction enzymes are site-specific endonucleases used in molecular cloning.

Step 2: Meaning

"Type IIP" specifically refers to those that recognize symmetric (palindromic) sequences and cut within or near that sequence.

Step 3: Analysis

Statement A is wrong because they are endonucleases (cut inside), not exonucleases (cut ends). Statements C and D are wrong because recognition depends on specific sequences, not just overall GC/AT content.

Step 4: Conclusion

Only Statement B—acting on palindromic sequences—is a defining characteristic of Type IIP enzymes. Final Answer: (B)
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