Question:
The level of expression of recombinant protein can be gauged by:
The level of expression of recombinant protein can be gauged by:
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Western Blot = Protein Detection. Northern = RNA. Southern = DNA.
Updated On: May 14, 2026
- number of transformants obtained.
- nutrient utilization rate.
- the level of resistance to antibiotic.
- Western blotting.
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The Correct Option is D
Solution and Explanation
Step 1: Concept
Assessing expression levels requires a technique that specifically detects and quantifies the target protein.
Step 2: Meaning
Western blotting is an analytical technique used to detect specific proteins in a sample.
Step 3: Analysis
Western blotting uses antibodies to bind to the recombinant protein after gel electrophoresis. The intensity of the resulting band directly correlates with the amount of protein present, allowing researchers to gauge expression levels. Antibiotic resistance only tells you if the plasmid is present.
Step 4: Conclusion
Western blotting is the standard method for verifying the successful translation and quantity of a recombinant protein. Final Answer: (D)
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