Question:
How many clones must you get to make a representative genomic library of a plant with a genome of a billion base pairs (bp), provided your vector carrying capacity is 10 kbp. Remember to allow a redundancy of at least one order of magnitude.
How many clones must you get to make a representative genomic library of a plant with a genome of a billion base pairs (bp), provided your vector carrying capacity is 10 kbp. Remember to allow a redundancy of at least one order of magnitude.
Show Hint
One order of magnitude = 10x. Always multiply your basic "coverage" by 10 to ensure a "representative" library.
Updated On: May 14, 2026
- Thousand
- Million
- Lakh
- Hundred
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The Correct Option is B
Solution and Explanation
Step 1: Concept
The number of clones ($N$) needed to represent a genome is roughly (Genome Size Vector Capacity) $\times$ Redundancy.
Step 2: Meaning
Genome = $10^9$ bp. Vector = $10 \times 10^3 = 10^4$ bp.
Step 3: Analysis
Base clones = $10^9 / 10^4 = 10^5$ (which is one Lakh). The question requires a redundancy of "one order of magnitude" (multiplying by 10). So, $10^5 \times 10 = 10^6$.
Step 4: Conclusion
$10^6$ is equal to one Million. Final Answer: (B)
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