Question:
Which of the following can be a restriction site for type II restriction enzyme?
Which of the following can be a restriction site for type II restriction enzyme?
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Restriction sites are usually 4-8 bp palindromes.
Updated On: May 14, 2026
- 5' AAGG 3' 3' TTCC 5'
- 3' AGTC 5' 5' TCAG 3'
- 5' GGCC 3' 3' CCGG 5'
- 5' ACCA 3' 3' TGGT 5'
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Verified By Collegedunia
The Correct Option is C
Solution and Explanation
Step 1: Concept
Type II restriction enzymes recognize specific DNA sequences and cut within or near them.
Step 2: Meaning
A hallmark of most Type II restriction sites is that they are palindromic sequences.
Step 3: Analysis
A palindromic sequence reads the same 5' to 3' on both strands.
• (A) 5' AAGG 3' vs 5' CCTT 3' (Not palindromic)
• (B) 5' TCAG 3' vs 5' CTGA 3' (Not palindromic)
• (C) 5' GGCC 3' vs 5' GGCC 3' (Palindromic)
Step 4: Conclusion
Option (C) is a perfect palindrome (specifically the site for HaeIII), making it a valid restriction site. Final Answer: (C)
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