List of top Questions asked in GAT-B- 2022

Regulation of lac operon is negative as:

During replication, DNA polymerases distinguish between ribonucleoside and deoxyribonucleoside triphosphates (rNTPs and dNTPs) while synthesizing new DNA strand. This is because:

A double-stranded genomic DNA has 40% (A+T) content. What is the average size (base pairs) of the fragments generated by digestion with the restriction enzyme HaeIII (GGCC)?

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R.
Assertion A: Attenuation of the trp operon is possible in bacteria.
Reason R: Transcription and translation are coupled.

The following diagram represents a restriction enzyme site:
Statement I: After digestion cohesive ends will be formed.
Statement II: These cohesive ends will have phosphate groups at 3' and 5' ends.

Which of the following can be a restriction site for type II restriction enzyme?

A mutant bacterial cell has a defective aminoacyl synthetase that attaches a lysine to tRNAs with the anticodon AAA instead of the normal phenylalanine. During protein synthesis:

Which is the correct order of progressively higher levels of chromatin organization?
A. Nucleosome
B. 30 nm chromatin fibre
C. Looped domain

Lac repressor of E. coli contains ________ DNA binding motifs.

Knockout mice are usually created by:

RNAi or post-transcriptional gene silencing is a conserved biological response to the presence of:

Which protein is required to seal a nick during Okazaki fragment maturation?

Self splicing introns are an example of:

Given below are two statements about hnRNA processing:
Statement I: Capping occurs by addition of methyl adenosine triphosphate to the 5' end.
Statement II: During tailing, about 200-300 adenylate residues are added to its 3' end.

The vector which is most commonly used in crop improvement is:

Given below are few statements related to plant tissue culture:
A. Regeneration frequencies of different types of explants derived from the same plant would always be identical.
B. A higher auxin: cytokinin ratio would induce production of roots under culture conditions.
C. Embryos can be obtained from somatic tissues of plants under in vitro culture conditions.
D. Since plants can photosynthesize, addition of a carbon source in tissue culture media is not advisable.

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R.
Assertion A: A linear DNA molecule containing three recognition sites for a restriction enzyme will generate multiple DNA fragments on partial digestion.
Reason R: Partial digestion by restriction enzymes leads to a heterogeneous population of DNA fragments.

Match items in List I with items in List II:

Match items List I with items in List II:

Simple sequence repeats (SSRs) are best utilized for:

Why is golden rice pale yellow in color?

Given below are two statements:
Statement I: After immobilization, plant cells are subjected to stress to induce secondary metabolite production.
Statement II: Cells derived from different explants in culture always produce the same set of secondary metabolites.

Given below are two statements:
Statement I: The fragments of DNA generated after cleavage with a restriction enzyme have a phosphate group on their 3' ends and a -OH group on their 5' ends.
Statement II: Cohesive ends generated by restriction digestion reanneal with complementary DNA fragment.

Given below are two statements:
Statement I: Virulence trait of Agrobacterium tumefaciens is borne on genomic DNA.
Statement II: The technique used to introduce genes in dicot is Ti-plasmid infection.

Given below are two statements:
Statement I: Bisexual flowers promote self-pollination.
Statement II: Self-incompatibility and male-sterility prevent self-pollination.