List of top Biochemistry Questions

An enzyme-catalyzed reaction is found to have \(\Delta G = -100\) kJ mol\(^{-1}\). Which of the following statements about this reaction is/are true?
You have purified an enzyme using a series of chromatographic methods. It was observed that a 10 \(\mu\)g mL-1 of this purified enzyme converted 10 mM substrate per hour at 25\(^{\circ}\)C and pH 7. Its specific activity is IU \(\mu\)g-1. (rounded off to three decimal places)
Which of the following enzyme is involved in the protection of cells from reactive oxygen species?
Which of the following carbohydrates is a NON-reducing sugar and therefore gives a NEGATIVE Benedict’s (and Fehling’s) test?
Which of the following enzymes is active in its dephosphorylated state?
Which technique is best suited for identifying a specific protein in a formalin-fixed paraffin-embedded (FFPE) tissue section?
High serum triglycerides are seen in all of the following conditions EXCEPT:
Ultraviolet (UV) radiation causes DNA damage primarily by which of the following mechanisms?
Which vitamin-derived coenzyme is required for a transamination reaction?
Define the following term: Glycosidic linkage.
Differentiate between the following:
(i) Acidic amino acids and basic amino acids
(ii) Nucleotide and Nucleoside
Define the following term: Invert sugar.
Define the following term: Anomers.
Assertion (A): The two strands of DNA are complementary to each other.
Reason (R): The hydrogen bonds are formed between specific pairs of bases.
An example of non-reducing sugar is
Differentiate between the following:

(i) Peptide linkage and Glycosidic linkage

(ii) Essential amino acids and Non-essential amino acids
Assertion (A): The pentaacetate of glucose does not react with \(H_2N-OH\).
Reason (R): It indicates the presence of free −CHO group in glucose.
The deficiency of which of the following vitamins causes increased fragility of red blood cells and muscular weakness?
Which of the following is an active form of thyroid hormone that acts through a nuclear receptor to stimulate energy-yielding metabolism by increasing the expression of genes encoding key catabolic enzymes?
Which of the following organs has both exocrine and endocrine functions?
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Intracellular receptors that act as transcription factors become activated by physiological ligands. Reason (R): They are activated in the interior of the cell (unlike extracellular receptors) because their physiological ligands are lipophilic in nature. In the light of the above statements, choose the most appropriate answer from the options given below:
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Tropic hormones act on other endocrine glands to stimulate the release of their hormones and transmit signalling through cell surface receptors. Reason (R): This action is attributed to their lipophilic nature. In the light of the above statements, choose the most appropriate answer from the options given below:
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Some cell signalling receptors are at the cell surface with transmembrane domains that help them embed in the plasma membrane. Reason (R): They are localized at the cell surface as their activating ligands cannot enter the cell interior. In the light of the above statements, choose the most appropriate answer from the options given below:
Arrange the following stages of spermatogenesis in proper sequence of sperm development: A. Spermatids, B. Spermatocytes, C. Spermatogonia, D. Spermatozoa Choose the correct answer from the options given below:
Arrange the following stages in sequence shown for human embryogenesis: A. Zygote, B. 8-cell stage, C. Gastrula, D. Blastula, E. Morula Choose the correct answer from the options given below: