Question

Given below are few statements related to plant tissue culture:
A. Regeneration frequencies of different types of explants derived from the same plant would always be identical.
B. A higher auxin: cytokinin ratio would induce production of roots under culture conditions.
C. Embryos can be obtained from somatic tissues of plants under in vitro culture conditions.
D. Since plants can photosynthesize, addition of a carbon source in tissue culture media is not advisable.

• A. A and C Only
• B. B and D Only
• C. B and C Only
• D. A and D Only

Hint:

Auxin = Rooting; Cytokinin = Shooting. (Think: A comes before C, Roots come before Shoots).

Answer 1

The Correct Option is C

Step 1: Concept
Plant tissue culture relies on the principles of totipotency and hormonal balance.

Step 2: Meaning
Auxins and cytokinins are the two main hormones that control organogenesis.

Step 3: Analysis
Statement A is false: explants (leaf, stem, root) have vastly different regeneration potentials. Statement B is true: high auxin leads to rooting. Statement C is true: this is called somatic embryogenesis. Statement D is false: in vitro plants have low photosynthetic rates and require sugar (sucrose) to grow.

Step 4: Conclusion
Statements B and C are the only scientifically correct ones. Final Answer: (C)