List of top Questions asked in GAT-B

Nick translation is carried out by
A single cell that can divide and give rise to a completely differentiated organism is referred to as
Which of the following statements are correct?
A. Mitochondria and chloroplast contain DNA and ribosomes for protein synthesis.
B. Mitochondria and chloroplast contain DNA but do not make protein.
C. These organelles are more related to bacteria/cyanobacteria than eukaryotes.
D. They are distinct entities and can survive outside the host cell.
E. They do not contain all the genes necessary to specify all their proteins.
A 5200-bp vector contains two BamHI sites 450-bp apart. A 1200-bp gene "X" with BamHI sites at ends is cloned after complete digestion. Size of the resultant plasmid?
Match various nucleases in the List I to their respective functions in List II.
The difference between a cosmid and a plasmid is the presence of which one of the following additional features in a cosmid?
Given three samples (a) L-Tyrosine (b) D-Tyrosine, (c) Equimolar mixture of L- and D-Tyrosine, which one of the following methods can help in the identification of the samples?
Calculate the approximate concentration of a solution with molar extinction coefficient = $650 \text{ M}^{-1}\text{cm}^{-1}$, Path length = 1 cm and Absorbance = 1.0.
From the purification table, what is the overall protein yield?


Identify the form of chromatography [Reverse Phase (RP) or Gel filtration (GF)] as shown in the chromatograms A and B below:
(A shows Protein eluting before Salt; B shows Salt eluting before Protein)
How much will 1 mole of glucose weigh (in grams) on the Earth (E) and on the Moon (M)?
What are the number of moles in 100 ml water (density = 1 g/ml) and in 100 ml heavy water (density = 1.11 g/ml) respectively?
Given the molecular weight of Glycine is 75, what would be the molecular weight of Gly-Gly dipeptide?
A student observed a decrease in Western blotting signal for PTEN in cancer cells but no change in Northern blotting signal. Which statement is correct?
Which one of the following is a receptor for HIV-1?
Zoonotic viruses are those that can be transmitted
Which one of the following DNA sequences can act as genetic markers?
Which of the following is NOT a genetic disorder?
The only genetically encoded amino acid without a stereoisomer is
Disulfide bonds are identified using the distance between the side-chains of which one of the following sulphur-containing residue pairs?
PSSM stands for
Which organ is used for isolation of activated B lymphocytes (plasma cells) for monoclonal antibody production?
The two strands in double-helical DNA are held together by hydrogen bonds between
Which one of the following attribute of a macromolecule is modulated by Molecular dynamics simulations?
Papain digestion of an IgG predominantly results in the formation of