List of top Questions asked in GAT-B

Match items in List I with items in List II

Which one of the following are Professional Antigen Presenting Cells?

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R.
Assertion A: Polyclonal antibodies bind to the same antigen but recognize different epitopes.
Reason R: Polyclonal antibodies are produced by different B cell clones in the body.

Identify the statement that is NOT applicable to enzyme catalyzed reaction.

Match items in List I with items in List II
List I: A. Cytochrome oxidase, B. DNA polymerase, C. Nitrate reductase, D. Urease
List II: I. Molybdenum, II. Nickel, III. Magnesium, IV. Copper

At the time of completion of protein synthesis, the sequence in which they leave the ribosome is:

NK cells are immunologically significant as they recognize and kill:

The primary function of the class I and class II MHC molecules is:

What is the advantage of using the neo gene to disrupt the function of a gene in knockout mice?

Subtractive hybridization is useful to:

Regulation of lac operon is negative as:

During replication, DNA polymerases distinguish between ribonucleoside and deoxyribonucleoside triphosphates (rNTPs and dNTPs) while synthesizing new DNA strand. This is because:

The percentage of sequence identity for the following aligned sequence is:
V K S F L W T Q A L
V P S F R W T Q S L

The following figure shows agarose gel electrophoresis of linear DNA, after digestion with BamHI in lane C and EcoRI in lane D. The uncut DNA is shown in lane B.
Statement I: This DNA has two restriction sites for BamHI and one restriction site for EcoRI.
Statement II: Double digestion with BamHI and EcoRI will yield 5 fragments.

The level of expression of recombinant protein can be gauged by:

Recombinants with insertional inactivation of $\beta$-galactosidase are identified by their inability to:

Which of the following can be a restriction site for type II restriction enzyme?

A mutant bacterial cell has a defective aminoacyl synthetase that attaches a lysine to tRNAs with the anticodon AAA instead of the normal phenylalanine. During protein synthesis:

A double-stranded genomic DNA has 40% (A+T) content. What is the average size (base pairs) of the fragments generated by digestion with the restriction enzyme HaeIII (GGCC)?

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R.
Assertion A: Attenuation of the trp operon is possible in bacteria.
Reason R: Transcription and translation are coupled.

The following diagram represents a restriction enzyme site:
Statement I: After digestion cohesive ends will be formed.
Statement II: These cohesive ends will have phosphate groups at 3' and 5' ends.

Which is the correct order of progressively higher levels of chromatin organization?
A. Nucleosome
B. 30 nm chromatin fibre
C. Looped domain

Self splicing introns are an example of:

Knockout mice are usually created by:

Lac repressor of E. coli contains ________ DNA binding motifs.