List of top Microbiology Questions

Which of the following are true ? A. All the monosaccharides except dihydroxyacetone contain one or more asymmetric carbon atoms.
B. Sucrose is a disaccharide consisting of D-glucose and D-fructose.
C. Sugars capable of reducing cupric ions are called reducing sugars.
D. Starch is the main storage polysaccharide of animal cells.
E. The rigid bacterial cell wall is made up of cellulose.
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Select correct statements regarding mutation. A. A point mutation involves substitution of a single nucleotide pair.
B. Frameshift mutations result from insertion or deletion of nucleotides not in multiples of 3.
C. Silent mutations always alter the amino acid sequence of a protein.
D. Mutagens can be physical, chemical or biological agents.
E. Auxotrophs can be easily grown on minimal media.
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Which of the following statement about Fungi are correct ? A. Dimorphic fungi can exist as unicellular yeast like as well as filamentous mold form.
B. Most fungi prefer an alkaline pH for growth.
C. Fungi are eukaryotic, spore bearing, photosynthetic organisms.
D. Conidiospores are sexual spores of fungi.
E. Some fungi can capture nematodes and kill them.
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Which of the following is correct about resolving power of a Microscope ? A. Limit of Resolution is directly proportional to the numerical aperture.
B. Resolution power of a microscope increases with magnification.
C. The greatest resolution in light microscopy is obtained with the shortest wavelength of light.
D. Limit of resolution is the smallest distance by which two objects can be separated and still be distinguishable.
E. The maximum resolution of a light microscope is \(0.02\, \mu m\).
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Pick the correct statements :
A. Restriction enzymes produce sticky or blunt ends.
B. DNA ligase forms disulfide bonds.
C. All plasmids act as cloning vectors.
D. Recombinant DNA can be expressed in a suitable host cell.
E. YACs are used for preparing human genome libraries.
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Which of the following statements on Proton gradient and ATP synthesis are correct ? A. Protons accumulate in the mitochondrial matrix.
B. PMF drives ATP synthesis.
C. ATP synthase is also called complex IV.
D. Chemiosmotic theory was proposed by Peter Mitchell.
E. ATP is synthesized during electron transfer through complexes.
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Choose correct statements about two-dimensional electrophoresis. A. It is a combination of IEF and SDS-PAGE.
B. It separates proteins of identical molecular weight that differ in pI.
C. It can't separate proteins with similar pI values but differ in molecular weight.
D. Thousands of cellular proteins can be resolved with this technique.
E. Microscopy can be used to identify individual protein spots in IEF.
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Which of the following statements is correct ? A. Intercalating agents distort DNA to induce single nucleotide pair insertions and deletions.
B. T and G typically exist in the amino form and A and C in the keto form.
C. Base analogues are structurally similar to normal nitrogenous bases.
D. Point mutations are mutations that occur at specific positions.
E. Nonsense mutations convert a sense codon to a stop codon.
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Which of the following statements about adaptive immunity are correct ? A. Adaptive immunity includes physical and chemical barriers to infection.
B. Adaptive immunity evolves in real time in response to infection.
C. Adaptive immunity response is highly diverse in specificity.
D. Major components of adaptive immunity are phagocytes.
E. Adaptive immune response has immunologic memory.
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Which of the following statements about the immune response are incorrect ? A. Neutrophils possess lobed nucleus.
B. Hormones are key mediators of an immune response.
C. Macrophages can be found in tissues throughout the body.
D. Eosinophils are the first cells to reach a site of infection.
E. The components of complement cascade are present in blood as inactive zymogens.
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Which of the following coenzymes/prosthetic groups are required by PDH complex for its functioning ? A. NAD
B. TPP
C. FMN
D. FAD
E. ATP
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Arrange the components of photosynthetic machinery of purple bacteria in a sequence followed for flow of electrons :
A. Quinone
B. P870
C. Cyt \(bc_1\) complex
D. Cyt \(c_2\)
E. Pheophytin
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Arrange the steps in cloning and expressing a gene in correct order :
A. Introduction of recombinant molecule into new host that expresses the gene
B. Use of restriction enzymes or PCR to generate fragments of DNA
C. Protein purification
D. Isolation of DNA to be cloned
E. Generation of a recombinant molecule by inserting DNA fragments into vectors
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