Question

Match List-I with List-II: 

List-I (Vector)		List-II (Special Feature)
(A)	pBR322	(III)	Ampicillin and Tetracycline Resistance
(B)	pUC Vector	(I)	LacZ α Gene for Blue-White Screening
(C)	Cosmids	(IV)	Efficient Packaging into Phage Particles
(D)	YAC	(II)	Centromere and Telomere

• A. \(A\text{-II},\ B\text{-IV},\ C\text{-I},\ D\text{-III}\)
• B. \(A\text{-III},\ B\text{-I},\ C\text{-II},\ D\text{-IV}\)
• C. \(A\text{-III},\ B\text{-I},\ C\text{-IV},\ D\text{-II}\)
• D. \(A\text{-I},\ B\text{-III},\ C\text{-IV},\ D\text{-II}\)

Hint:

Remember:
• pBR322 \(\rightarrow\) Ampicillin + Tetracycline resistance
• pUC vectors \(\rightarrow\) LacZ \(\alpha\) blue-white screening
• Cosmids \(\rightarrow\) Phage packaging
• YAC \(\rightarrow\) Centromere and telomeres

Answer 1

The Correct Option is C

Concept: Different cloning vectors possess unique features that make them useful for specific molecular biology applications. Common vectors include:
• Plasmids
• Cosmids
• YACs

Step 1: Matching pBR322. pBR322 contains genes for: \[ \text{Ampicillin resistance} \] and \[ \text{Tetracycline resistance} \] used as selectable markers. Thus: \[ A \rightarrow III \]

Step 2: Matching PUC vector. PUC vectors contain: \[ \text{LacZ }\alpha \text{ gene} \] which is used in: \[ \text{Blue-white screening} \] Thus: \[ B \rightarrow I \]

Step 3: Matching Cosmids. Cosmids possess: \[ \cos \text{ sites} \] allowing: \[ \text{Efficient packaging into phage particles} \] Thus: \[ C \rightarrow IV \]

Step 4: Matching YAC. YACs (Yeast Artificial Chromosomes) contain:
• Centromere
• Telomeres allowing stable maintenance in yeast cells. Thus: \[ D \rightarrow II \]

Step 5: Determining the correct combination. Therefore: \[ \boxed{ A\text{-III},\ B\text{-I},\ C\text{-IV},\ D\text{-II} } \] Hence, the correct answer is: \[ \boxed{(C)\ A\text{-III},\ B\text{-I},\ C\text{-IV},\ D\text{-II}} \]