Question:
Which of the following exhibits maximum mobility in an agarose gel?
Which of the following exhibits maximum mobility in an agarose gel?
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• Supercoiled $>$ Linear $>$ Nicked (mobility)
• More compact = Faster movement
Updated On: May 13, 2026
- Nicked Plasmid DNA
- Supercoiled Plasmid DNA
- Linear Plasmid DNA
- Single stranded Plasmid DNA
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The Correct Option is B
Solution and Explanation
Concept:
In agarose gel electrophoresis, DNA mobility depends on size, shape, and conformation.
• More compact structures move faster.
• Less compact or relaxed structures move slower.
Step 1: Analyzing DNA forms.
• Nicked plasmid DNA: Relaxed circular form → Slowest mobility.
• Supercoiled plasmid DNA: Highly compact → Fastest mobility.
• Linear plasmid DNA: Intermediate mobility.
• Single stranded DNA: Less compact and behaves differently → slower than supercoiled.
Step 2: Selecting the correct answer.
Since supercoiled DNA is most compact, it shows maximum mobility in agarose gel.
• More compact structures move faster.
• Less compact or relaxed structures move slower.
Step 1: Analyzing DNA forms.
• Nicked plasmid DNA: Relaxed circular form → Slowest mobility.
• Supercoiled plasmid DNA: Highly compact → Fastest mobility.
• Linear plasmid DNA: Intermediate mobility.
• Single stranded DNA: Less compact and behaves differently → slower than supercoiled.
Step 2: Selecting the correct answer.
Since supercoiled DNA is most compact, it shows maximum mobility in agarose gel.
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