Question:
Which of the following could be a recognition site of a Type II restriction endonuclease?
Which of the following could be a recognition site of a Type II restriction endonuclease?
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Restriction enzymes recognize palindromes:
Example: GAATTC (EcoRI)
Updated On: May 13, 2026
- $5'$-TTAACCGGAA-$3'$
- $5'$-GCTAATAGC-$3'$
- $5'$-TTTAATTAAA-$3'$
- $5'$-CCCTATAGG-$3'$
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The Correct Option is C
Solution and Explanation
Concept:
Type II restriction endonucleases recognize palindromic DNA sequences, meaning the sequence reads the same in the 5' $\rightarrow$ 3' direction on both strands.
Step 1: Check for palindromic sequence.
A palindromic sequence satisfies: \[ \text{Sequence} = \text{Reverse complement} \]
Step 2: Analyze options.
• (A) Not palindromic
• (B) Not palindromic
• (C) Palindromic → matches reverse complement
• (D) Not palindromic
Step 3: Final conclusion.
Thus, the correct recognition site is: \[ $5'$\texttt{-TTTAATTAAA-$3'$} \]
Step 1: Check for palindromic sequence.
A palindromic sequence satisfies: \[ \text{Sequence} = \text{Reverse complement} \]
Step 2: Analyze options.
• (A) Not palindromic
• (B) Not palindromic
• (C) Palindromic → matches reverse complement
• (D) Not palindromic
Step 3: Final conclusion.
Thus, the correct recognition site is: \[ $5'$\texttt{-TTTAATTAAA-$3'$} \]
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