Question:
Which of the following is generally NOT a step in PCR?
Which of the following is generally NOT a step in PCR?
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• PCR steps: Denaturation → Annealing → Extension
• Ligation → Used in cloning, not PCR
Updated On: May 13, 2026
- Denaturation
- Elongation
- Annealing
- Ligation
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The Correct Option is D
Solution and Explanation
Concept:
Polymerase Chain Reaction (PCR) involves three main cyclic steps:
• Denaturation: Separation of double-stranded DNA into single strands.
• Annealing: Binding of primers to complementary sequences.
• Extension (Elongation): DNA synthesis by DNA polymerase.
Step 1: Analyzing each option.
• Denaturation: Essential PCR step → Correct step.
• Elongation: DNA synthesis step → Correct step.
• Annealing: Primer binding step → Correct step.
• Ligation: Joining DNA fragments using ligase → Not part of PCR.
Step 2: Selecting the correct answer.
PCR does not involve ligation, so the correct answer is (D).
• Denaturation: Separation of double-stranded DNA into single strands.
• Annealing: Binding of primers to complementary sequences.
• Extension (Elongation): DNA synthesis by DNA polymerase.
Step 1: Analyzing each option.
• Denaturation: Essential PCR step → Correct step.
• Elongation: DNA synthesis step → Correct step.
• Annealing: Primer binding step → Correct step.
• Ligation: Joining DNA fragments using ligase → Not part of PCR.
Step 2: Selecting the correct answer.
PCR does not involve ligation, so the correct answer is (D).
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