Question:

Which of the following is NOT a multiple sequence alignment program?

Show Hint


• CLUSTAL family + PILEUP → Alignment tools
• AMBER → Molecular dynamics, not alignment
Updated On: May 13, 2026
  • CLUSTALX
  • AMBER
  • CLUSTALW
  • PILEUP
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The Correct Option is B

Solution and Explanation

Concept: Multiple Sequence Alignment (MSA) programs are used to align three or more biological sequences (DNA, RNA, or protein) to identify similarities and evolutionary relationships.
• Common MSA tools include CLUSTALX, CLUSTALW, and PILEUP.
• Some software tools serve different purposes like molecular dynamics simulations.

Step 1:
Analyzing each option.

CLUSTALX: A widely used graphical interface for multiple sequence alignment → MSA program.
AMBER: A molecular dynamics simulation software used for biomolecular modeling → Not an MSA program.
CLUSTALW: A widely used multiple sequence alignment tool → MSA program.
PILEUP: A program used for sequence alignment → MSA program.

Step 2:
Identifying the correct answer.
Only AMBER is not a multiple sequence alignment program.
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