List of top Plant Biotechnology Questions

Which are widely used techniques to fuse plant protoplasts?
A. Chemical fusion by PEG
B. Electric stimulation
C. High $Ca^{2+}$ -high pH method
D. Particle gun method
Choose the correct answer from the options given below:

The steps involved in the process of somatic hybridization are:
A. Regeneration of hybrid plants
B. Fusion of the protoplasts of desired species/varieties
C. Isolation of protoplasts
D. Identification and selection of somatic hybrid cells
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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): The hybrid cells formed by the fusion of two unrelated diploid protoplasts combine a set of three genomes from the parents.
Reason (R): Partial or complete elimination of nuclear genome before regeneration leads to the formation of cybrid plants.
In the light of the above statements, choose the most appropriate answer from the options given below:

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): One of the major limitations of genetic engineering is the difficulty in transferring polygenic traits in plants.
Reason (R): Cybridization generates novel combinations of nuclear and/or cytoplasmic genomes, which is not possible through sexual hybridization.
In the light of the above statements, choose the most appropriate answer from the options given below:

Mention the correct sequence of events for the isolation of protoplasts from embryogenic suspension cultures of rice.
A. Excise yellow/white compact and nodular embryogenic callus
B. Staining with fluorescein diacetate
C. Filter the cell suspension through a nylon sieve
D. Filter the protoplast suspension through sieves
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Which of the following secondary metabolites is not commercially produced in vitro in a plant cell culture system?

Which technique is not used for the analysis of hybrid cells formed by the fusion of two unrelated protoplasts?

In most of the somatic hybridization events in plants, which cells are preferred to be used?

Which of the following culture type is the most suitable for the generation of somaclonal variations?

Which of the following is used for the visual assessment of percentage viability of protoplasts?

Match List - I with List - II.

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Match List - I with List - II during the process of androgenesis in plant.

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The osmotically fragile protoplasts look completely spherical under microscope in which of the following solution?

Match List I with List - II.

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Which of the following mechanism is not causing somaclonal variations?

Doubling of the chromosome number may be achieved by:
A. Use of spindle inhibitors
B. Marker-assisted breeding
C. Fusion of unreduced gametes
D. Protoplast fusion
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Gametoclonal variations from changes in chromosome number/structure may arise due to which of the following:
A. Polyploidy
B. Aneuploidy
C. Translocations and inversions
D. Reduction division
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The correct sequence of producing androgenic haploids of Nicotiana tabacum is:
A. Anthers burst to release the pollen derived embryo
B. Collection of flower buds
C. Isolation and growth of plantlets emerging from the anthers
D. Placing the anthers on the MS medium
Choose the correct answer from the options given below:

For the production of mulberry triploid plants
A. Wash the roots of plants and transfer them to small pots
B. Culture on MS medium containing BAP and NAA
C. Seed surface sterilization
D. Excision of endosperm
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Haploid embryos are produced in vivo by:
A. Karyogenesis
B. Parthenogenesis
C. Meiosis
D. Pseudogamy
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Haploid production in barley following interspecific hybridization usually involves:
A. Pollen collection and cross pollination
B. Embryo culture
C. Emasculation
D. Colchicine treatment
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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): Chromosome number may change under prolonged plant tissue culture regimes.
Reason (R): Endosperm cells are triploid in nature.
In the light of the above statements, choose the most appropriate answer from the options given below:

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): Gynogenic haploids have been developed by determining the optimum stage of male gametophytic development.
Reason (R): Late uninculeate to trinucleate stage of megaspore development is preferred for the production of gynogenic haploids.
In the light of the above statements, choose the most appropriate answer from the options given below:

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): Haploid plants can be produced through in vitro culture of plant gametic cells.
Reason (R): Accurate phenotyping in plant breeding with double haploidy is possible because of their segregating nature.
In the light of the above statements, choose the most appropriate answer from the options given below:

How much sucrose is generally required for the initiation of androgenesis in plants?