Question

The correct sequence of producing androgenic haploids of Nicotiana tabacum is:
A. Anthers burst to release the pollen derived embryo
B. Collection of flower buds
C. Isolation and growth of plantlets emerging from the anthers
D. Placing the anthers on the MS medium
Choose the correct answer from the options given below:

• A. B, A, D, C
• B. C, A, B, D
• C. B, D, A, C
• D. A, D, B, C

Hint:

Think of the lab flow: Pick (Buds) $\rightarrow$ Plate (Media) $\rightarrow$ Pop (Anthers burst) $\rightarrow$ Pick (Plantlets).

Answer 1

The Correct Option is C

Concept: The production of haploids from tobacco (Nicotiana tabacum) is one of the most classic and successful protocols in plant tissue culture. It follows a logical laboratory workflow.

Step 1: Preparation and Inoculation.
The process starts in the field or greenhouse with the Collection of flower buds (B) at the correct developmental stage (usually when the petals are just emerging from the calyx). These buds are surface sterilized.

Step 2: Culture Initiation.
The anthers are then carefully dissected out of the buds and placed into the culture environment—specifically Placing the anthers on the MS medium (D).

Step 3: Development and Regeneration.
After a few weeks of incubation, the microspores inside the anther develop into embryos. Eventually, the Anthers burst to release the pollen-derived embryos (A). These small structures then grow into distinct plants, leading to the final step: the Isolation and growth of plantlets (C) into full haploid tobacco plants.