Question

For the production of mulberry triploid plants
A. Wash the roots of plants and transfer them to small pots
B. Culture on MS medium containing BAP and NAA
C. Seed surface sterilization
D. Excision of endosperm
Choose the correct answer from the options given below:

• A. D, B, A, C
• B. C, D, B, A
• C. B, D, A, C
• D. A, B, D, C

Hint:

To get a Triploid ($3n$), go to the Endosperm. The sequence is always: Clean the seed $\rightarrow$ Take the endosperm $\rightarrow$ Grow it $\rightarrow$ Pot it.

Answer 1

The Correct Option is B

Concept: Triploid plants ($3n$) are often desirable in agriculture (like mulberry) because they are often seedless, have larger leaves, or possess higher vigor. A common way to produce them in vitro is through endosperm culture, as the endosperm is naturally triploid tissue.

Step 1: Initial Preparation.
The process must begin with the source material—the seeds. To ensure a sterile environment for tissue culture, the first step is Seed surface sterilization (C).

Step 2: Tissue Isolation and Inoculation.
Once sterilized, the seeds are dissected to perform the Excision of endosperm (D). This triploid tissue is then placed onto a nutrient-rich environment for growth, specifically Culture on MS medium containing BAP and NAA (B), where these hormones trigger callogenesis or direct regeneration.

Step 3: Hardening and Transfer.
After the plants have successfully regenerated in the lab, they must be transitioned to the real world. This final stage involves the Wash the roots of plants and transfer them to small pots (A) for hardening.