Question

Haploid production in barley following interspecific hybridization usually involves:
A. Pollen collection and cross pollination
B. Embryo culture
C. Emasculation
D. Colchicine treatment
Choose the correct answer from the options given below:

• A. A, B, D, C
• B. C, A, D, B
• C. A, D, C, B
• D. C, A, B, D

Hint:

The Barley Bulbosum Method is a "Sneaky" way to get haploids: Cross them, let the plant delete the extra chromosomes, and then rescue the survivor!

Answer 1

The Correct Option is D

Concept: The "Bulbosum method" in barley involves crossing common barley (Hordeum vulgare) with Hordeum bulbosum. After fertilization, the H. bulbosum chromosomes are eliminated, leaving a haploid embryo.

Step 1: Hybridization Prep.
First, to prevent self-pollination in the female parent, Emasculation (C) is performed. This is followed by the actual crossing through Pollen collection and cross pollination (A) using the second species.

Step 2: Rescue and Doubling.
Because these hybrid embryos often abort on the plant due to chromosome elimination, they must be saved via Embryo culture (B) in a lab. Once a haploid plantlet is formed, its fertility is restored via Colchicine treatment (D) to double the chromosomes.