List of top Biotechnology Questions asked in GAT-B

Immobilization of enzymes:
A. increases the specificity of the enzyme in batch reaction
B. leads to an increase in the apparent $K_{m}$
C. makes it unsuitable for its use in a continuous reactor system
D. decrease the operational cost of the industrial process

Which of the following represents the correct sequence of MAP kinase signal transduction pathway?
A. Ras, B. Raf, C. ERK, D. Mek

A peptide containing only aromatic amino acids (tryptophan, phenylalanine, and tyrosine) has a molecular weight of 11,000. The likely number of amino acid residues in the peptide is:

Match items in List I with items in List II
List I (Enzyme): A. Cytochrome oxidase, B. DNA polymerase, C. Nitrate reductase, D. Urease
List II (Cofactor): I. Molybdenum, II. Nickel, III. Magnesium, IV. Copper

What is the advantage of using the neo gene to disrupt the function of a gene in knockout mice?

RNAi or post-transcriptional gene silencing is a conserved biological response to the presence of:

Knockout mice are usually created by:

The vector which is most commonly used in crop improvement is:

Match items in List I with items in List II:

Given below are few statements related to plant tissue culture:
A. Regeneration frequencies of different types of explants derived from the same plant would always be identical.
B. A higher auxin: cytokinin ratio would induce production of roots under culture conditions.
C. Embryos can be obtained from somatic tissues of plants under in vitro culture conditions.
D. Since plants can photosynthesize, addition of a carbon source in tissue culture media is not advisable.

Given below are two statements:
Statement I: Virulence trait of Agrobacterium tumefaciens is borne on genomic DNA.
Statement II: The technique used to introduce genes in dicot is Ti-plasmid infection.

Why is golden rice pale yellow in color?

Given below are two statements:
Statement I: After immobilization, plant cells are subjected to stress to induce secondary metabolite production.
Statement II: Cells derived from different explants in culture always produce the same set of secondary metabolites.

During protoplast fusion, the product has the nucleus of one of the parents in which the chromosome elimination took place in subsequent cell division and the cytoplasm was contributed by both the parents. Such a product will be called:

Which one of the following small RNAs in plants DO NOT regulate gene expression by cleavage of mRNAs?

Assertion A: Bt toxin does not kill Bacillus thuringiensis.
Reason R: The Bt toxin gets activated due to the alkaline pH of the insect gut.

Lung cancer can be detected at an early stage by:

The primary enzyme responsible for the rapid and accurate replication of the genome in E. coli is:

The limit of resolution of a light microscope is determined by:

Which of the following is true for single cell protein?

The Cartagena Protocol regulates trade in

In a batch experiment with cells following Monod growth kinetics, the initial substrate concentration is doubled from $S_0$ to $2S_0$ and it is observed that the initial doubling time in the log phase hardly changes. This tells us that

Which one of the following products is obtained by a trans-esterification step starting from sugarcane bagasse?

Select the INCORRECT statement from the following options.

In an ideal Continuous Stirred Tank Reactor (CSTR) at steady state