Question

The limit of resolution of a light microscope is determined by:

• A. wavelength of light
• B. material of the lens
• C. polarization of light
• D. intensity of light

Hint:

This is why electron microscopes have better resolution—electrons have much shorter wavelengths than light.

Answer 1

The Correct Option is A

Step 1: Concept
Resolution is the ability to distinguish two closely spaced points as separate entities.

Step 2: Meaning
According to Abbe's equation, resolution limit $d = \lambda / (2 \cdot NA)$, where $\lambda$ is wavelength and $NA$ is numerical aperture.

Step 3: Analysis
The smaller the wavelength of the light used, the smaller the distance $d$ that can be resolved, thereby increasing the resolution.

Step 4: Conclusion
Thus, the wavelength of light is the fundamental limiting factor for resolution in optical microscopy. Final Answer: (A)