List of practice Questions

How is CML detected by FISH techniques?
Enlist any two limitations of batch culture.
Even if we know where the genes are present in a genome, it is not entirely clear how to count them. Give two reasons for this problem.
Briefly explain the process of sickling of Red Blood Cells (RBCs) during sickle cell anaemia. Why does sickle celled RBCs lead to anaemic conditions in patients?
How does the metagenomics approach helps to identify novel genes present in an environment? Explain the process.
What is the use of introducing the property of delayed fruit ripening in some plants? Explain the methods used for delaying fruit ripening.
Mention the catalytic triad (along with diagram) which contributes to the enzymatic activity of native subtilisin. Why was this enzyme improved by protein engineering? Write the method used in this process.
Since many centuries, whey has been used for the treatment of numerous ailments. Briefly elaborate upon the scientific relevance of it.
Selection methods are based on the expression or non-expression of certain traits in recombinant microbial cells. Enlist such any three traits.
Explain briefly the procedures of the following techniques used in recombinant DNA Technology:
(i) Transfection
(ii) Electroporation
(iii) Biolistics
On which plasmid are Bacterial Artificial Chromosome (BAC) vectors based? Enlist the important sequences present in these vectors.
Given below is a single stranded sequence of DNA:
5' ACTAGAATTCGCCA 3'
Which restriction enzyme would cleave this sequence in double stranded form? Illustrate the fragments that will be generated upon digestion by this restriction enzyme.
Although not required for cell growth, antibiotics are one of the most essential components of animal cell culture medium. Why? Give examples of two such antibiotics often used in animal cell culture medium.
What is meant by 'Gene knock out'? How is it useful in developing mouse models for studying human diseases?
Why is an inverted microscope required to observe animal cell cultures?
How is chymotrypsinogen converted to chymotrypsin in the duodenum? What is the name of this process?
SCID (Severe Combined Immuno Deficiency) is caused due to deficiency of which enzyme?
Assertion (A): The human embryonic stem cells can be derived from the inner cell mass of blastocyst and cultured in a suitable nutrient medium.
Reason (R): It is possible to selectively remove a gene and make precise genetic modification in mouse embryonic stem cells.
Assertion (A): Mutation selection technique identifies micro organisms that have been improved by induced mutations.
Reason (R): Mutations cannot be induced by irradiations in micro-organisms.
Assertion (A): M13 bacteriophage uses rolling circle mechanism for DNA replication.
Reason (R): During rolling circle mechanism, double stranded replicative form of DNA is formed which replicates until there are about its hundred copies in the host bacterial cell.
Assertion (A): The gene that encodes for Barnase is used to induce male sterility in transgenic plants.
Reason (R): Barnase is a RNA hydrolyzing enzyme that inhibits pollen formation in the tapetal cells of anther.
The natural defence mechanism of bacteria occurs for:
The technique of Nick Translation was developed by:
Locus link was primarily developed to:
Which of the following is a function of collagen in human body?