Question:
The technique of Nick Translation was developed by:
The technique of Nick Translation was developed by:
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Nick translation relies on the dual activity of E. coli DNA Polymerase I: its 5' to 3' exonuclease activity degrades the existing unlabeled DNA strand from a nick, while its 5' to 3' polymerase activity simultaneously synthesizes a new labeled strand.
Updated On: Jun 19, 2026
- Rigby and Paul Berg in 1877
- Rigby and Paul Berg in 1977
- Linus Pauling in 1985
- Paul Berg in 1973
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The Correct Option is B
Solution and Explanation
Step 1: Concept
Nick translation is an in vitro method used to introduce radioactively or fluorescently labeled nucleotides into double-stranded DNA molecules to generate highly sensitive molecular probes.
Step 2: Historical Development
This enzymatic labeling technique was first developed and described by Peter Rigby and Paul Berg in the year 1977.
Step 3: Elimination
Option (A) is chronologically impossible (DNA's structure was not even known in 1877). Paul Berg's classic recombinant DNA experiments occurred in the early 1970s, but the specific Nick Translation protocol was officially published in 1977.
Step 4: Conclusion
Hence, the correct combination of scientists and date is Rigby and Paul Berg in 1977, matching option (B). Final Answer: (B)
Nick translation is an in vitro method used to introduce radioactively or fluorescently labeled nucleotides into double-stranded DNA molecules to generate highly sensitive molecular probes.
Step 2: Historical Development
This enzymatic labeling technique was first developed and described by Peter Rigby and Paul Berg in the year 1977.
Step 3: Elimination
Option (A) is chronologically impossible (DNA's structure was not even known in 1877). Paul Berg's classic recombinant DNA experiments occurred in the early 1970s, but the specific Nick Translation protocol was officially published in 1977.
Step 4: Conclusion
Hence, the correct combination of scientists and date is Rigby and Paul Berg in 1977, matching option (B). Final Answer: (B)
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