Question:
Sterility testing of antibiotic-active-containing parenteral preparations that inhibit microbial growth is performed by which method
Sterility testing of antibiotic-active-containing parenteral preparations that inhibit microbial growth is performed by which method
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Filter first, wash away the "inhibitor" (antibiotic), then grow the "trapped" germs.
Updated On: May 25, 2026
- Membrane filtration method
- Pour plate method
- Direct inoculation method
- Spread plate method
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The Correct Option is A
Solution and Explanation
Step 1: Concept
Sterility testing ensures that a pharmaceutical product is free from viable microorganisms.
Step 2: Meaning
Preparations containing antibiotics possess inherent antimicrobial activity that can cause false-negative results in sterility tests.
Step 3: Analysis
In membrane filtration, the product is passed through a filter that traps microbes. The antibiotic is then washed away with a sterile diluent before the filter is incubated in growth media. Direct inoculation would leave the antibiotic in the media, inhibiting microbial growth.
Step 4: Conclusion
Membrane filtration is the preferred method for antibiotics as it allows for the removal of inhibitory substances. Final Answer: (A)
Sterility testing ensures that a pharmaceutical product is free from viable microorganisms.
Step 2: Meaning
Preparations containing antibiotics possess inherent antimicrobial activity that can cause false-negative results in sterility tests.
Step 3: Analysis
In membrane filtration, the product is passed through a filter that traps microbes. The antibiotic is then washed away with a sterile diluent before the filter is incubated in growth media. Direct inoculation would leave the antibiotic in the media, inhibiting microbial growth.
Step 4: Conclusion
Membrane filtration is the preferred method for antibiotics as it allows for the removal of inhibitory substances. Final Answer: (A)
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