Question:
Where can an alien DNA be cloned or made into multiple copies?
Where can an alien DNA be cloned or made into multiple copies?
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Ori is the mandatory site for DNA replication in plasmids and vectors.
Updated On: Jun 20, 2026
- Chromosome
- Telomere
- Centromere
- Origin of Replication
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The Correct Option is D
Solution and Explanation
Step 1: Understanding DNA cloning requirement.
For any DNA molecule (including alien DNA) to be copied, it must have a specific site where replication can begin. This site is essential for initiation of DNA replication.
Step 2: Definition of Origin of Replication (Ori).
The origin of replication is a specific nucleotide sequence where DNA replication machinery binds and starts replication.
Step 3: Role in recombinant DNA technology.
When alien DNA is inserted into a host, it will replicate only if it contains or is linked to an origin of replication so that host enzymes can copy it.
Step 4: Evaluating other options.
- Chromosome: Entire DNA structure, not a replication initiation site.
- Telomere: Protects chromosome ends, not involved in cloning.
- Centromere: Helps chromosome segregation, not replication initiation.
Step 5: Logical reasoning.
Without Ori, DNA cannot replicate, so cloning is impossible. Therefore Ori is essential for multiple copies.
Step 6: Final conclusion.
Hence, alien DNA cloning occurs at the origin of replication.
For any DNA molecule (including alien DNA) to be copied, it must have a specific site where replication can begin. This site is essential for initiation of DNA replication.
Step 2: Definition of Origin of Replication (Ori).
The origin of replication is a specific nucleotide sequence where DNA replication machinery binds and starts replication.
Step 3: Role in recombinant DNA technology.
When alien DNA is inserted into a host, it will replicate only if it contains or is linked to an origin of replication so that host enzymes can copy it.
Step 4: Evaluating other options.
- Chromosome: Entire DNA structure, not a replication initiation site.
- Telomere: Protects chromosome ends, not involved in cloning.
- Centromere: Helps chromosome segregation, not replication initiation.
Step 5: Logical reasoning.
Without Ori, DNA cannot replicate, so cloning is impossible. Therefore Ori is essential for multiple copies.
Step 6: Final conclusion.
Hence, alien DNA cloning occurs at the origin of replication.
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