Question

What is the advantage of using ddNTPs conjugated with fluorescent molecules in automated method of DNA sequencing?

Hint:

Fluorescent ddNTPs enable fast, safe, and precise automated DNA sequencing by allowing all bases to be detected simultaneously in one reaction.

Answer 1

Using ddNTPs conjugated with fluorescent molecules in automated DNA sequencing offers several advantages:

• Simultaneous detection: Different fluorescent dyes attached to each ddNTP allow all four nucleotides to be identified in a single reaction mixture.
• High throughput and speed: Automated systems detect fluorescence as DNA fragments pass through a laser detector, enabling rapid sequencing.
• Increased accuracy: Fluorescence signals provide clear, precise identification of terminating bases, reducing errors.
• Safety and convenience: Eliminates the need for radioactive labeling and manual gel reading.
• Automated data analysis: Fluorescence data can be processed by software for quick and accurate base calling.