Question:
__________ of the following is not adsorbent for thin layer chromatography.
__________ of the following is not adsorbent for thin layer chromatography.
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The three classic, standard stationary phase adsorbents used in Thin Layer Chromatography (TLC) are Silica Gel, Alumina, and Cellulose.
Updated On: Jul 22, 2026
- Silica gel
- Alumina
- Cellulose powder
- Protein powder
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The Correct Option is D
Solution and Explanation
Step 1: Understanding the Concept:
Thin Layer Chromatography (TLC) is a separation technique where the stationary phase is applied as a thin layer on a flat, inert support plate (glass, plastic, or aluminum).
The stationary phase must act as an effective adsorbent, separating analytes based on their varying partition coefficients or adsorption strengths.
Step 2: Detailed Explanation:
Let us evaluate each of the listed stationary phase materials:
1. Silica gel (A): The most widely used polar adsorbent in TLC. It contains active silanol (\(-\text{Si-OH}\)) groups that separate molecules based on polarity.
2. Alumina (Aluminum Oxide) (B): A highly popular, polar, basic or acidic adsorbent used for separating basic compounds, alkaloids, and organic pigments.
3. Cellulose powder (C): Acts primarily as a support for a partition system (retaining water molecules), highly useful for separating hydrophilic compounds like amino acids, sugars, and food dyes.
4. Protein powder (D): Proteins are macromolecules with complex, variable, and unstable folded structures. They do not possess the uniform pore size, thermal stability, or chemical inertness required to act as a standardized adsorbent stationary phase for thin layer chromatography.
Therefore, protein powder is not used as a chromatography adsorbent.
Step 3: Final Answer:
Thus, Protein powder is not an adsorbent for thin layer chromatography.
Thin Layer Chromatography (TLC) is a separation technique where the stationary phase is applied as a thin layer on a flat, inert support plate (glass, plastic, or aluminum).
The stationary phase must act as an effective adsorbent, separating analytes based on their varying partition coefficients or adsorption strengths.
Step 2: Detailed Explanation:
Let us evaluate each of the listed stationary phase materials:
1. Silica gel (A): The most widely used polar adsorbent in TLC. It contains active silanol (\(-\text{Si-OH}\)) groups that separate molecules based on polarity.
2. Alumina (Aluminum Oxide) (B): A highly popular, polar, basic or acidic adsorbent used for separating basic compounds, alkaloids, and organic pigments.
3. Cellulose powder (C): Acts primarily as a support for a partition system (retaining water molecules), highly useful for separating hydrophilic compounds like amino acids, sugars, and food dyes.
4. Protein powder (D): Proteins are macromolecules with complex, variable, and unstable folded structures. They do not possess the uniform pore size, thermal stability, or chemical inertness required to act as a standardized adsorbent stationary phase for thin layer chromatography.
Therefore, protein powder is not used as a chromatography adsorbent.
Step 3: Final Answer:
Thus, Protein powder is not an adsorbent for thin layer chromatography.
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