Mechanism-Based Recall: Induced breeding needs a hormone that can trigger the pituitary to release its OWN spawning hormones — narrow down by that mechanism.
GnRH analogues (like Ovaprim/Ovatide) stimulate the fish's pituitary gland to release gonadotropins, directly inducing spawning outside the natural season.
Insulin and thyroxine have no link to the reproductive-hormone pathway, and melatonin only influences seasonal rhythm cues, not direct spawning induction.