Question

Match List - I with List - II.

Choose the correct answer from the options given below:

• A. A-I, B-II, C-III, D-IV
• B. A-III, B-I, C-IV, D-II
• C. A-IV, B-III, C-II, D-I
• D. A-IV, B-III, C-I, D-II

Hint:

MS Media Cheat Sheet: Nicotinic Acid = Vitamin; Casein = Nitrogen; Cytokinin = Shoots; Auxin = Callus/Roots.

Answer 1

The Correct Option is C

Concept: Success in plant tissue culture depends on the precise composition of the culture media, such as the Murashige and Skoog (MS) medium. Each component serves a specific nutritional or regulatory function.

Step 1: Vitamins and Nitrogen Sources.
Nicotinic acid (A) is a essential vitamin (IV) commonly added to MS media to act as a co-enzyme in metabolic reactions. Casein hydrolysate (C) is a mixture of amino acids derived from milk protein, serving as an excellent source of organic nitrogen (II) that is easily assimilated by cultured cells.

Step 2: Hormonal Regulation.
Cytokinins (B) are primarily responsible for promoting shoot induction (III) and axillary bud proliferation. Auxins (D) are the key regulators for the induction of cell division and callus formation (I), as well as root initiation.

Step 3: Conclusion.
Matching the components to their roles gives the sequence: A-IV, B-III, C-II, D-I.