Question

Select the correct sequence of steps involved in the isolation of protoplasts from the mesophyll cells of a plant leaf:
A. Culture of protoplast suspension
B. Infiltration of the enzyme into the leaf tissue
C. Incubate leaf pieces in filter-sterilized enzyme solution
D. Release of mesophyll protoplasts in solution
Choose the correct answer from the options given below:

• A. B, C, D, A
• B. A, D, C, B
• C. C, B, D, A
• D. C, D, B, A

Hint:

Think of the process as: Inject the enzymes $\rightarrow$ Wait (Incubate) $\rightarrow$ Collect (Release) $\rightarrow$ Grow (Culture).

Answer 1

The Correct Option is C

Step 1: Understanding the Question:

We have to sequence the steps of protoplast isolation from leaf mesophyll cells.

Step 2: Detailed Explanation:

• Leaf pieces are placed in a filter-sterilized enzyme solution (C).

• The enzyme is infiltrated into the leaf tissue (B), usually with gentle vacuum, so that it reaches the mesophyll cells.

• The cell walls get digested and mesophyll protoplasts are released into the solution (D).

• Finally the purified protoplast suspension is cultured (A).

• Sequence: C, B, D, A.

Step 3: Final Answer:
The correct option is (C) C, B, D, A.