Question

During gel electrophoresis migration of DNA fragments leading to their separation takes place on the agarose gel. Choose the correct option:

• A. The smaller the fragment size, the farther it moves.
• B. Positively charged fragments moves to farther end.
• C. The larger the fragment size, the farther it moves.
• D. The negatively charged fragments do not move.

Hint:

In gel electrophoresis, smaller DNA fragments migrate faster and farther than larger ones.

Answer 1

The Correct Option is A

Step 1: DNA is negatively charged and moves towards the positive electrode in electrophoresis.
Step 2: Smaller DNA fragments pass more easily through the agarose matrix, moving farther than larger ones.
Step 3: Therefore, separation is based on size: smaller = farther.