Question

Which of the following can be used for cell-line authentication?

• A. Serotyping
• B. Virus infection
• C. PCR
• D. Protein estimation

Hint:

• STR-PCR → Standard for cell line authentication
• DNA methods $>$ Protein methods for identity

Answer 1

The Correct Option is C

Concept: Cell-line authentication ensures the identity and purity of cultured cells.
• Molecular techniques are commonly used
• DNA-based methods provide high specificity

Step 1: Understanding authentication methods.

• PCR-based methods (e.g., STR profiling) are widely used for identifying cell lines

Step 2: Analyzing options.

• Serotyping: Not specific for cell line identity → Incorrect.
• Virus infection: Not an authentication method → Incorrect.
• PCR: DNA-based identification → Correct.
• Protein estimation: Measures quantity, not identity → Incorrect.

Step 3: Selecting the correct answer.
Thus, PCR is used for cell-line authentication.