Question

The step followed during DNA profiling in which DNA sample is subjected to restriction endonuclease is .................

• A. Hybridization
• B. DNA isolation
• C. DNA amplification
• D. DNA fragmentation

Hint:

Restriction endonucleases play a key role in DNA profiling by fragmenting the DNA at specific sites, creating a unique pattern.

Answer 1

The Correct Option is D

Step 1: Understanding DNA profiling.
In DNA profiling, the DNA sample is subjected to restriction endonuclease enzymes, which cleave the DNA into smaller fragments for further analysis. This step is called DNA fragmentation.
Step 2: Analyzing the options.
(A) Hybridization: Incorrect, hybridization involves the bonding of complementary DNA strands, not fragmentation.
(B) DNA isolation: Incorrect, DNA isolation is the process of extracting DNA from the cells, before fragmentation.
(C) DNA amplification: Incorrect, DNA amplification is done by PCR to increase the amount of DNA, but it is not related to restriction enzymes.
(D) DNA fragmentation: Correct, restriction endonuclease enzymes break DNA into fragments, a critical step in DNA profiling.
Step 3: Conclusion.
The correct answer is (D) DNA fragmentation, as this involves breaking down the DNA sample using restriction enzymes.