Question:
The primer extension during PCR cycle requires the temperature range of
The primer extension during PCR cycle requires the temperature range of
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Primer extension by Taq polymerase takes place at about 72 degrees Celsius.
Updated On: Nov 13, 2026
- \(70 - 75^{\circ}\)C
- \(90 - 98^{\circ}\)C
- \(65 - 85^{\circ}\)C
- \(35 - 40^{\circ}\)C
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The Correct Option is A
Solution and Explanation
Step 1: Understanding the Concept
A PCR cycle has three steps: denaturation, annealing and extension.
Step 2: Temperatures
Denaturation: about \(94\text{-}98^{\circ}\)C.
Annealing of primers: about \(40\text{-}60^{\circ}\)C.
Extension by Taq polymerase: about \(72^{\circ}\)C.
Step 3: Match
The extension range \(70\text{-}75^{\circ}\)C is option (A).
Final Answer:
Primer extension needs about \(70\text{-}75^{\circ}\)C. \[ \boxed{\text{(A) }70 - 75^{\circ}\text{C}} \]
A PCR cycle has three steps: denaturation, annealing and extension.
Step 2: Temperatures
Denaturation: about \(94\text{-}98^{\circ}\)C.
Annealing of primers: about \(40\text{-}60^{\circ}\)C.
Extension by Taq polymerase: about \(72^{\circ}\)C.
Step 3: Match
The extension range \(70\text{-}75^{\circ}\)C is option (A).
Final Answer:
Primer extension needs about \(70\text{-}75^{\circ}\)C. \[ \boxed{\text{(A) }70 - 75^{\circ}\text{C}} \]
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