Question

The Rolling tube technique is used for cultivation of:

• A. Algae
• B. Thermophilic bacteria
• C. Stringent anaerobes
• D. Microaerophiles

Hint:

Remember:
• Hungate Rolling tube technique \(\rightarrow\) Cultivation of strict anaerobes
• Strict anaerobes \(\rightarrow\) Cannot tolerate oxygen
• Reducing agents help maintain anaerobic conditions \[ \text{Rolling tube method} = \text{Anaerobic microbiology technique} \]

Answer 1

The Correct Option is C

Concept: The Rolling Tube Technique, also known as the Hungate technique, is a microbiological method used for the cultivation of strict or stringent anaerobic microorganisms. These organisms cannot tolerate oxygen because oxygen is toxic to them. Therefore, they must be grown under completely oxygen-free conditions. The technique involves:
• Use of anaerobic culture medium
• Removal of oxygen using reducing agents
• Rolling the tube to form a thin agar layer inside the tube
• Maintaining an oxygen-free environment

Step 1: Understanding the purpose of the Rolling Tube Technique. Strict anaerobes require: \[ \text{Complete absence of oxygen} \] The rolling tube method helps in:
• Isolation of anaerobic bacteria
• Growth under reduced oxygen conditions
• Preventing oxygen exposure during cultivation Therefore: \[ \boxed{\text{Rolling tube technique is used for cultivation of stringent anaerobes}} \]

Step 2: Eliminating the incorrect options.
• Option (A): Algae are generally cultivated using light-exposed aquatic culture methods.
• Option (B): Thermophilic bacteria require high temperatures, not specifically rolling tube cultivation.
• Option (D): Microaerophiles require low oxygen concentration but not completely anaerobic conditions. Hence, the correct answer is stringent anaerobes. \[ \boxed{\text{Rolling tube technique} \Rightarrow \text{Strict anaerobic cultivation}} \]