Concept:
RT-PCR (Reverse Transcription Polymerase Chain Reaction) is used in forensic biology to detect tissue-specific mRNA markers. Different body fluids express specific genes, which help in their identification.
Step 1: Understanding salivary markers.
Saliva contains specific proteins and enzymes that are encoded by particular genes. These genes can be detected using RT-PCR.
Step 2: Evaluating Histatin 3 (C).
Histatin proteins are salivary proteins involved in antimicrobial activity. Histatin 3 is a well-known marker for saliva.
Thus, C is correct.
Step 3: Evaluating Statherin (D).
Statherin is another salivary protein that helps in maintaining calcium balance in saliva. It is also a recognized marker.
Thus, D is correct.
Step 4: Evaluating other options.
• Kallikrein 3 – Associated with prostate-specific antigen (PSA), not saliva.
• Spsherin – Not a standard salivary marker.
Step 5: Final evaluation.
Thus, the correct combination is C and D.
Final Conclusion:
Hence, the correct answer is option (4).