Question:

The expression of the two RT-PCR based analysis can be used for identification of salivary ? A. Kallikrein 3
B. Spsherin
C. Histatin 3
D. Statherin
Choose the correct answer from the options given below :

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Saliva markers → Histatin & Statherin; PSA markers → Prostate-related.
Updated On: May 18, 2026
  • A and B Only
  • B and D Only
  • A and C Only
  • C and D Only
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The Correct Option is D

Solution and Explanation

Concept: RT-PCR (Reverse Transcription Polymerase Chain Reaction) is used in forensic biology to detect tissue-specific mRNA markers. Different body fluids express specific genes, which help in their identification.

Step 1: Understanding salivary markers.

Saliva contains specific proteins and enzymes that are encoded by particular genes. These genes can be detected using RT-PCR.

Step 2: Evaluating Histatin 3 (C).

Histatin proteins are salivary proteins involved in antimicrobial activity. Histatin 3 is a well-known marker for saliva.
Thus, C is correct.

Step 3: Evaluating Statherin (D).

Statherin is another salivary protein that helps in maintaining calcium balance in saliva. It is also a recognized marker.
Thus, D is correct.

Step 4: Evaluating other options.


• Kallikrein 3 – Associated with prostate-specific antigen (PSA), not saliva.
• Spsherin – Not a standard salivary marker.

Step 5: Final evaluation.

Thus, the correct combination is C and D. Final Conclusion:
Hence, the correct answer is option (4).
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