Question:
Study the diagram given below that shows the steps involved in the procedure of selecting transformed bacteria and answer the questions that follow:
(a) Identify the colony that has got transformed. Justify your answer.
Study the diagram given below that shows the steps involved in the procedure of selecting transformed bacteria and answer the questions that follow:
(a) Identify the colony that has got transformed. Justify your answer.
(a) Identify the colony that has got transformed. Justify your answer.
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In transformation experiments:
- Disrupted resistance gene = Loss of resistance.
- Growth indicates retained resistance (e.g., ampicillin resistance).
Updated On: Jan 13, 2026
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Solution and Explanation
- The transformed colony is the one that grows on Plate M (with ampicillin) but does not grow on Plate N (with both ampicillin and tetracycline).
- Justification: The recombinant DNA disrupts the tetracycline resistance gene, making the bacteria sensitive to tetracycline, but retains ampicillin resistance, allowing growth on Plate M.
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